Generating Aldehyde-Tagged Antibodies
Summary: This article is a selection of articles published in BioMed Central Ltd., giving overview on the ability to site-specifically conjugate a protein to a payload of interest has found widespread application in basic research and drug development.
The team set out to achieve consistently high yields by modulating culture conditions to maximize FGE activity within the cell. They recently showed that FGE is a copper-dependent oxidase that binds copper in a stoichiometric fashion and uses it to activate oxygen, driving enzymatic turnover. Building upon that work, this Research Article shows that by supplementing cell culture media with copper they can routinely reach high yields of highly converted protein.